We use cookies to deliver the best service to you. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium … A zwitterionic N-substituted aminosulfonic acid buffer. HEPES solution, you enter 062K1064. A zwitterionic N-substituted aminosulfonic acid buffer. Lower concentrations are usually not adequate to control fluctuations in pH, and higher concentrations are often toxic. The 100 mL size is also sold separately (catalog number CC-5022). WHERE SCIENCE INTERSECTS INNOVATIONTM. HEPES BSS at a fill volume of 100 mL is a component of the ReagentPack TM Subculture kit (catalog number CC-5024). At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. All Rights Reserved. Has a pKa of 7.55 at 25°C. HEPES Buffered Saline Solution for use in cell culture, 500 mL. HEPES Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid) is a commonly used zwitterionic buffer with a pKa of 7.3 at 37 °C. Need larger quantities for your development, manufacturing or research applications? HEPES Buffer Solution is a biological buffer used in cell culture media. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. search was unable to find the COA you are looking for, you will be Bio-Techne 1 x Bottle HEPES Buffered Solution, 500 mL. OmniPur HEPES, Sodium Salt - CAS 75277-39-3 - Calbiochem, |  Match Criteria: Product Name, Property. Reproduction of any materials from the site is strictly forbidden without permission. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO, Internal Search Keywords: HEPES buffer|HEPES solution|HIPS solution|HIPIS solution|HEEPS|7200, Tissue and Cell Culture Dissociation Reagents. HEPES buffer solution 1 M in H 2 O Synonym: HEPES solution, N-(2-Hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) CAS Number 7365-45-9. Liver Cells for ADME & Toxicology Studies, Endotoxin Detection and Analysis Software. HEPES finds its usage in in vitro experiments on Mg. HEPES has been used:• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum• To prepare differentiation medium for PC12 cells. | Privacy. eCl@ss 32129211 . We offer a 100% guarantee on all our products. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Site Use Terms Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. There are currently no reviews for this product. © 2020  Merck KGaA, Darmstadt, Germany and/or its affiliates. Since high levels of HEPES can cause cytotoxicity, concentrations of this organic buffer should be reduced if toxicity is apparent for a specific cell line or primary cell culture. The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications. It is typically used in cell culture at concentration between 5mM to 30 mM. Na, Synonym: Since the buffering capacity of HEPES is independent of the CO2 concentration, it is an ideal buffer for maintaining the pH of cultures when cell cultures require extended periods of manipulation outside of a CO2 incubator. You must have JavaScript enabled in your browser to utilize the functionality of this website. Has a pKa of 7.48 at 25°C. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt, Anti-phospho-AMPA Glutamate Receptor 1 (pSer. More important for COO: enter a "0" if only two numbers are JavaScript seems to be disabled in your browser. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt. The addition of 1025 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incub Customer Service, Synonym: PubChem Substance ID 57652951. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. Be the first to HEPES Buffer Solution is used as a common buffer in cell culture media. HEPES has been described as one of the best all-purpose buffers available for biological research. CAS : 7365-45-9 : Grade : CLIA/ultra pure : Formula weight : 238.31 : Formula : C8H18N2O4S : Physical description HEPES may be added to cell culture media at a final concentration of 10mM to 25 mM to provide additional buffering capacity if required. All Rights Reserved. Grab a coffee and check out our free webinars. The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid-d, PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration), BioUltra, for molecular biology, ≥99.5% (T), Pharmaceutical Secondary Standard; Certified Reference Material, BioPerformance Certified, suitable for cell culture, ≥99.5%, ≥99.5% (titration), free-flowing, Redi-Dri, 1M, BioReagent, suitable for cell culture, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered. Absorbance (1.0 mM, H2O, 260 nm):0.10. A buffer solution of HEPES can be prepared by any of several methods. The 100 mL size is also sold separately (catalog number CC-5022). The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications. HEPES Buffer Solution is a biological buffer used in cell culture media. It is commonly used to buffer cell culture media in air. To prepare 1L of 1M HEPES buffer, you need: ... because it seems that if I analysed the another 500 μl of the same stock buffer solution, it seems a completely different buffer, and it starts absorbing. presented with a COA Request form. Add some to cart or login. If you find a lot number with a filling-code such as Materials. D609, Empirical Formula (Hill Notation): C11H15KOS2. Contact © 2020  Merck KGaA, Darmstadt, Germany and/or its affiliates. HEPES BSS at a fill volume of 100 mL is a component of the ReagentPackTM Subculture kit (catalog number CC-5024). The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many oth… 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt. $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image, $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers.